Data/hora: 01/06/2024 06:18:17

Provedor de dados: | 58
País: | Brazil
Título: | Detection of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil
Autores: | Alves,Clebert José; Figueiredo,Salomão Moreira de; Azevedo,Sérgio Santos de; Clementino,Inácio José; Keid,Lara Borges; Vasconcellos,Sílvio Arruda; Batista,Carolina de Sousa Américo; Rocha,Vivianne Cambuí Mesquita; Higino,Severino Silvano.
Data: | 2010-06-01
Ano: | 2010
Palavras-chave: | Brucella ovis; Isolation; PCR; Ovine; Paraíba State.
Resumo: | To determine the presence of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil, 80 animals slaughtered in the public slaughterhouse of Patos city were used. Before slaughter, blood samples were collected by jugular venopuncture from each animal, and after slaughter, testicles, epidydimus and uterus were aseptically collected. For the serological diagnosis of B. ovis and B. abortus infections, the agar gel immunodiffusion (AGID) and Rose Bengal (RBT) tests were carried out, respectively. In addition, microbiological culture and polymerase chain reaction (PCR) were performed on testicle, epidydimus and uterus samples. Six animals (7.5%) tested positive for the presence of B. ovis antibodies and all animals tested negative for the presence of B. abortus antibodies. One AGID-positive animal tested positive at uterine swab culture. PCR was able to amplify DNA of Brucella spp. from the pool of testicle, epidydimus and uterus samples from AGID-positive animals. This is the first report of isolation and detection of B. ovis DNA by PCR in ovine from the Northeast region of Brazil.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200016
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822010000200016
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.41 n.2 2010
Fonte: | info:eu-repo/semantics/openAccess