Data/hora: 11/06/2024 09:22:51

Provedor de dados: | 58
País: | Brazil
Título: | Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques
Autores: | Vieira-da-Motta,Olney; Folly,Márcio Manhães; Sakyiama,Cássia Camargo Hagen.
Data: | 2001-03-01
Ano: | 2001
Palavras-chave: | Bovine mastitis; Staphylococcus aureus; Coagulase; PCR.
Resumo: | Contamination of fresh milk with Staphylococcus aureus was assessed comparatively through routine phenotypic (coagulase tube test and coagulase slide test) and genotypic (PCR) screening of 128 S. aureus strains isolated from 555 milk samples. These samples were collected from 362 cows with subclinical mastitis, hosted in different dairy herds at various locations of the Northern and Northeastern rural areas of the State of Rio de Janeiro, 39.7% of which were CMT-positive. All S. aureus isolates tested positive for the presence of the coagulase gene by PCR and the isolates could be grouped into four distinct classes according to the size of the PCR product. The strains also yielded variable results when assayed with coagulase test. Taken together, these data indicate the existence of extensive polymorphism at the coagulase gene locus in the genus Staphylococcus and exemplifies the extent of molecular and phenotypic heterogeneity associated with the strains circulating in rural herds.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100007
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822001000100007
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.32 n.1 2001
Fonte: | info:eu-repo/semantics/openAccess