Data/hora: 11/06/2024 01:21:55

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Cloning and expression of Aujeszky's disease virus glycoprotein E (gE) in a baculovirus system	
Autores: 	Dambros,Régia Maria Feltrin; Ribeiro,Bergman Moraes; Aguiar,Raimundo Wagner de S.; Schaefer,Rejane; Esteves,Paulo Augusto; Perecmanis,Simone; Simon,Neide Lisiane; Silva,Nayara Cavalcante; Coldebella,Michele; Ciacci-Zanella,Janice Reis.	
Data: 	2007-09-01	
Ano: 	2007	
Palavras-chave: 	Aujeszky; Glycoprotein E; Baculovírus; Recombinant.	
Resumo: 	Aujeszky' s disease (AD) is an infectious disease causing important economic losses to the swine industry worldwide. The disease is caused by an alpha-herpesvirus, Aujeszky' s disease virus (ADV), an enveloped virus with a double stranded linear DNA genome. The ADV genome encodes 11 glycoproteins, which are major targets for the immune system of the host in response to the infection. The glycoprotein E (gE) is a non-essential protein and deletion of the gE gene has been used for the production of marker vaccines. Aiming to develop molecular reagents for the production of a gE specific ELISA test, the gE gene was amplified by PCR, cloned and expressed into a baculovirus expression system. The recombinant DNA vector pFastBac-gE-ADV was used for site-specific transposition into the recombinant baculovirus (bacmid). Colonies with recombinant bacmid-pFastBac-gE-ADV were selected by antibiotic and color selection and the presence of the gE gene was confirmed by PCR. The recombinant bacmid-pFastBac-gE-ADV was cotransfected in insect Trichoplusia ni and the presence of the recombinant DNA and gE protein were detected by PCR, SDS-PAGE and Western blotting, respectively.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300021	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822007000300021	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.38 n.3 2007	
Fonte: 	info:eu-repo/semantics/openAccess