Data/hora: 11/06/2024 12:06:22

Provedor de dados: | 58
País: | Brazil
Título: | Characterization of recombinant MSP5 Anaplasma marginale Havana isolate
Autores: | Corona,B.; Machado,H.; Rodríguez,M.; Martínez,S.
Data: | 2009-12-01
Ano: | 2009
Palavras-chave: | Anaplasma marginale; Major surface proteins; Characterization; Havana isolate; HMSP5.
Resumo: | Anaplasma marginale is the causative agent of bovine anaplasmosis, a disease of worldwide economic importance. Major surface proteins (MSPs) are involved in host-pathogen and tick-pathogen interactions and they have been used as markers for the genetic characterization of A. marginale strains and phylogenetic studies. The major surface protein 5 (MSP5) is highly conserved in the genus Anaplasma and in all isolates of A. marginale. The aim of the present work was to carry out the cloning, sequencing and characterization of the recombinant MSP5 Anaplasma marginale Havana isolate. The sequence of the msp5 gene of Anaplasma marginale Havana isolate with a size of 633 pb was determined (Acc. No. AY527217). This gene was cloned into pRSETB vector and expressed in Escherichia coli. The MSP5 protein was recognized by the monoclonal antibody ANAF16C1 and it showed a high similitude percent with the gene sequence described for other Anaplasma marginale isolates. These data are very important for the development of a diagnostic test for A. marginale using the MSP5 recombinant protein.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400032
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822009000400032
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.40 n.4 2009
Fonte: | info:eu-repo/semantics/openAccess