Data/hora: 09/06/2024 07:21:45

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria	
Autores: 	Süle,Judit; Kõrösi,Tímea; Hucker,Attila; Varga,László.	
Data: 	2014-09-01	
Ano: 	2014	
Palavras-chave: 	Mupirocin; Clindamycin; Ciprofloxacin; Bifidobacterium; Lactobacillus acidophilus.	
Resumo: 	The purpose of this study was to test the suitability of Transgalactosylated oligosaccharides-mupirocin lithium salt (TOS-MUP) and MRS-clindamycin-ciprofloxacin (MRS-CC) agars, along with several other culture media, for selectively enumerating bifidobacteria and lactic acid bacteria (LAB) species commonly used to make fermented milks. Pure culture suspensions of a total of 13 dairy bacteria strains, belonging to eight species and five genera, were tested for growth capability under various incubation conditions. TOS-MUP agar was successfully used for the selective enumeration of both Bifidobacterium animalis subsp. lactis BB-12 and B. breve M-16 V. MRS-CC agar showed relatively good selectivity for Lactobacillus acidophilus, however, it also promoted the growth of Lb. casei strains. For this reason, MRS-CC agar can only be used as a selective medium for the enumeration of Lb. acidophilus if Lb. casei is not present in a product at levels similar to or exceeding those of Lb. acidophilus. Unlike bifidobacteria and coccus-shaped LAB, all the lactobacilli strains involved in this work were found to grow well in MRS pH 5.4 agar incubated under anaerobiosis at 37 °C for 72 h. Therefore, this method proved to be particularly suitable for the selective enumeration of Lactobacillus spp.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300035	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822014000300035	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.45 n.3 2014	
Fonte: 	info:eu-repo/semantics/openAccess