Data/hora: 06/06/2024 06:07:06

Provedor de dados: | 58
País: | Brazil
Título: | Distribution of Hepatitis C virus (HCV) genotypes in seropositive patients in the state of Alagoas, Brazil
Autores: | Gonzaga,Rosa Maria S.; Rodart,Itatiana F.; Reis,Mitermayer Galvão; Ramalho Neto,Cícero Eduardo; Silva,Denise Wanderlei.
Data: | 2008-12-01
Ano: | 2008
Palavras-chave: | Hepatitis C virus; 5'NCR; Core region; RT-nested-PCR; Genotypes.
Resumo: | We determined the frequency of hepatitis C virus (HCV) genotypes in anti-HCV seropositive patients in the state of Alagoas, Brazil, by means of nested-reverse transcription-polymerase chain reaction (RT-nested-PCR) followed by restriction fragment length polymorphism (RFLP) of amplified fragments of the 5´NCR. The nested-PCR with genotype-specific primers from the core region was carried out when detection was not possible by the first approach. Detectable HCV-RNA was present in 115 (74.7%) of 154 serum samples. Genotype 1 was the most frequent (77.4%), against 20.9% of genotype 3 and 0.8% of genotype 2. Subtype 1b was predominant (65.2%), followed by subtypes 1a (8.7%), and 3a (6.1%). Coinfection (1a/3a) was detected in 0.8% of the samples. Indeed, there was no significant differences in the prevalence of genotype 1 compared to what has been obtained from anti-HCV seropositive patients from other locations in Brazil. Here we report for the first time the genotype 2 in the state of Alagoas.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400008
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822008000400008
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.39 n.4 2008
Fonte: | info:eu-repo/semantics/openAccess