Data/hora: 06/06/2024 05:56:30

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain	
Autores: 	Li,Jiang; Hu,Qiushi; Li,Yuquan; Xu,Yuan.	
Data: 	2015-09-01	
Ano: 	2015	
Palavras-chave: 	Psychrophilic bacteria; Cold-adapted agarase; Purification; Characterization.	
Resumo: 	An extracellular β-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Bay sediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight of 80 kDa. The optimum pH and temperature of the agarase were 8.0 and 30 °C, respectively. However, it maintained as much as 85% of the maximum activities at 10 °C. Significant activation of the agarase was observed in the presence of Mg2+, Mn2+, K+; Ca2+, Na+, Ba2+, Zn2+, Cu2+, Co2+, Fe2+, Sr2+ and EDTA inhibited the enzyme activity. The enzymatic hydrolyzed product of agar was characterized as neoagarobiose. Furthermore, this work is the first evidence of cold-adapted agarase in Antarctic psychrophilic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300683	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-838246320131289	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.46 n.3 2015	
Fonte: 	info:eu-repo/semantics/openAccess