Data/hora: 07/06/2024 09:40:44

Provedor de dados: | 58
País: | Brazil
Título: | Purification and characterization of cold-adapted beta-agarase from an Antarctic psychrophilic strain
Autores: | Li,Jiang; Hu,Qiushi; Li,Yuquan; Xu,Yuan.
Data: | 2015-09-01
Ano: | 2015
Palavras-chave: | Psychrophilic bacteria; Cold-adapted agarase; Purification; Characterization.
Resumo: | An extracellular β-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Bay sediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight of 80 kDa. The optimum pH and temperature of the agarase were 8.0 and 30 °C, respectively. However, it maintained as much as 85% of the maximum activities at 10 °C. Significant activation of the agarase was observed in the presence of Mg2+, Mn2+, K+; Ca2+, Na+, Ba2+, Zn2+, Cu2+, Co2+, Fe2+, Sr2+ and EDTA inhibited the enzyme activity. The enzymatic hydrolyzed product of agar was characterized as neoagarobiose. Furthermore, this work is the first evidence of cold-adapted agarase in Antarctic psychrophilic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300683
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-838246320131289
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.46 n.3 2015
Fonte: | info:eu-repo/semantics/openAccess