Data/hora: 11/06/2024 10:02:56

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Thermotolerant and mesophylic fungi from sugarcane bagasse and their prospection for biomass-degrading enzyme production	
Autores: 	Santos,Bruna Silveira Lamanes dos; Gomes,Arthur Filipe Sousa; Franciscon,Emanuele Giuliane; Oliveira,Jean Maikon de; Baffi,Milla Alves.	
Data: 	2015-09-01	
Ano: 	2015	
Palavras-chave: 	Β-glucosidase; Β-xylosidase; Xylanase; Aspergillus; Sugarcane bagasse.	
Resumo: 	Nineteen fungi and seven yeast strains were isolated from sugarcane bagasse piles from an alcohol plant located at Brazilian Cerrado and identified up to species level on the basis of the gene sequencing of 5.8S-ITS and 26S ribosomal DNA regions. Four species were identified: Kluyveromyces marxianus, Aspergillus niger, Aspergillus sydowii and Aspergillus fumigatus, and the isolates were screened for the production of key enzymes in the saccharification of lignocellulosic material. Among them, three strains were selected as good producers of hemicellulolitic enzymes: A. niger (SBCM3), A. sydowii (SBCM7) and A. fumigatus (SBC4). The best β-xylosidase producer was A. niger SBCM3 strain. This crude enzyme presented optimal activity at pH 3.5 and 55 °C (141 U/g). For β-glucosidase and xylanase the best producer was A. fumigatus SBC4 strain, whose enzymes presented maximum activity at 60 °C and pH 3.5 (54 U/g) and 4.0 (573 U/g), respectively. All these crude enzymes presented stability around pH 3.0–8.0 and up to 60 °C, which can be very useful in industrial processes that work at high temperatures and low pHs. These enzymes also exhibited moderate tolerance to ethanol and the sugars glucose and xylose. These similar characteristics among these fungal crude enzymes suggest that they can be used synergistically in cocktails in future studies of biomass conversion with potential application in several biotechnological sectors.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300903	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-838246320140393	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.46 n.3 2015	
Fonte: 	info:eu-repo/semantics/openAccess