Data/hora: 09/06/2024 02:45:57

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Production of recombinant flagellin to develop ELISA-based detection of Salmonella Enteritidis	
Autores: 	Mirhosseini,Seyed Ali; Fooladi,Abbas Ali Imani; Amani,Jafar; Sedighian,Hamid.	
Data: 	2017-12-01	
Ano: 	2017	
Palavras-chave: 	Salmonella Enteritidis; Flagellin C; Indirect ELISA; Bacterial detection; Food contaminated.	
Resumo: 	ABSTRACT Food-borne diseases, caused by the pathogenic bacteria, are highly prevalent in the world. Salmonella is one of the most important bacterial genera responsible for this. Salmonella Enteritidis (SE) is one of the non-typhoid Salmonellae that can be transmitted to human from poultry products, water, and contaminated food. In recent years, new and rapid detection methods such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) have been developed. In this study, recombinant FliC (rFliC) was produced to be used as an antigen. The immunization was conducted in mice with the purified recombinant FliC (rFliC). The mice were subcutaneously immunized with rFliC and elicited significant rFliC specific serum IgG antibodies. An indirect ELISA system was established for the detection of Salmonella Enteritidis. Our results confirmed that the recombinant flagellin can be one of the excellent indicators for the detection of Salmonella Enteritidis.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400774	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1016/j.bjm.2016.04.033	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.48 n.4 2017	
Fonte: 	info:eu-repo/semantics/openAccess