Data/hora: 05/06/2024 17:13:42

Provedor de dados: | 58
País: | Brazil
Título: | Genotypic characterization of psittacid herpesvirus isolates from Brazil
Autores: | Luppi,Marcela Miranda; Luiz,Ana Paula Moreira Franco; Coelho,Fabiana Magalhães; Ecco,Roselene; Fonseca,Flávio Guimarães da; Resende,Mauricio.
Data: | 2016-03-01
Ano: | 2016
Palavras-chave: | Psittacid herpesvirus; PstI; RFLP; PCR.
Resumo: | Abstract Thirty-six isolates of psittacid herpesvirus (PsHV), obtained from 12 different species of psittacids in Brazil, were genotypically characterized by restriction fragment length polymorphism (RFLP) analysis and PCR amplification. RFLP analysis with the PstI enzyme revealed four distinct restriction patterns (A1, X, W and Y), of which only A1 (corresponding to PsHV-1) had previously been described. To study PCR amplification patterns, six pairs of primers were used. Using this method, six variants were identified, of which, variants 10, 8, and 9 (in this order) were most prevalent, followed by variants 1, 4, and 5. It was not possible to correlate the PCR and RFLP patterns. Twenty-nine of the 36 isolates were shown to contain a 419 bp fragment of the UL16 gene, displaying high similarity to the PsHV-1 sequences available in GenBank. Comparison of the results with the literature data suggests that the 36 Brazilian isolates from this study belong to genotype 1 and serotype 1.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100217
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1016/j.bjm.2015.11.017
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.47 n.1 2016
Fonte: | info:eu-repo/semantics/openAccess