Data/hora: 11/06/2024 08:02:35

Provedor de dados: | 58
País: | Brazil
Título: | HCMV gB genotypes in cervical secretion and placenta tissues in the state of Espírito Santo, Southeastearn Brazil
Autores: | Spano,Liliana Cruz; Ferreira,Mônica Simões Rocha; Almeida,Marilda Santos; Nascimento,Jussara Pereira do; Leite,José Paulo Gagliardi.
Data: | 2007-09-01
Ano: | 2007
Palavras-chave: | Cytomegalovirus; RFLP; GB genotypes; Cervical secretion; Placenta tissues.
Resumo: | Human cytomegalovirus (HCMV) displays genetic variability in several regions, supposed to be related with strain-specific tissue tropism and immunopathogenesis. Based on sequence variation in the UL55 gene that encodes gB glycoprotein, HCMV strains can be assigned to one of four genotypes. Previous studies have addressed gB genotyping mostly by investigating strains derived from immunosuppressed patients, sometimes without previous knowledge about genotype distribution in a geographic area. The present study verified the distribution of HCMV gB genotypes of strains obtained from immunocompetent women at Vitória City, Espírito Santo State, Southeastern, Brazil. The HCMV genome was extracted from their cervical secretion, fetal and maternal placenta tissues (chorionic villous and decidua) from abortion cases and from white blood cells (WBCs). HCMV genotyping was performed by restriction fragment length polymorphism analyses of amplified product from the high variability site of the UL55 gene. All four genotypes were observed in both cervical secretion and placenta, whereas in WBCs a single gB1 genotype was detected. HCMV gB1 and gB2 genotypes were detected, respectively, in nine and in six of the 23 studied samples, while gB3 and gB4 were each found in four separate samples of the total. The differences in genotype frequency were not considered statistically significant. No mixed genotype infection was observed. The results indicated that the four gB HCMV genotypes had no particular tropism for placenta tissues and that all genotypes circulated within immunocompetent women at the time and in the region of study.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300008
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822007000300008
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.38 n.3 2007
Fonte: | info:eu-repo/semantics/openAccess