Data/hora: 10/06/2024 13:53:39

Provedor de dados: | 58
País: | Brazil
Título: | Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189
Autores: | Naz,Sehar Afshan; Jabeen,Nusrat; Sohail,Muhammad; Rasool,Sheikh Ajaz.
Data: | 2015-12-01
Ano: | 2015
Palavras-chave: | Bacteriocin; Pyocin; P. aeruginosa.
Resumo: | Abstract Pseudomonas aeruginosa, in spite of being a ubiquitous organism (as it is found in soil, water, and humans), is also an opportunistic pathogen. In order to maintain its diversity in the community, it produces various toxic proteins, known as, bacteriocins. In the present study, pyocin SA189, which is a bacteriocin produced by P. aeruginosa SA189 (isolated from a clinical sample) was characterized. P. aeruginosa SA189, as identified by the conventional and 16S rRNA gene amplification, produced pyocin SA189 of molecular weight of 66 k Da. The pyocin showed antimicrobial activity against several clinically relevant Gram-positive and Gram-negative bacteria and was substantially stable for wide ranges of temperature and pH. Furthermore, the pyocin also retained its biological activity upon treatment with metal ions, organic solvents, and various proteolytic and lipolytic enzymes. The data from the growth kinetics indicated that the maximum bacteriocin production occurred in the late log phase. Overall, our results signify the potential of pyocin SA189 as a bio-control agent.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401147
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-838246420140737
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.46 n.4 2015
Fonte: | info:eu-repo/semantics/openAccess