Data/hora: 09/06/2024 04:01:00

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Structure analysis of capsid protein of Porcine circovirus type 2 from pigs with systemic disease	
Autores: 	Gava,Danielle; Serrão,Vitor Hugo Balasco; Fernandes,Lana Teixeira; Cantão,Mauricio Egídio; Ciacci-Zanella,Janice Reis; Morés,Nelson; Schaefer,Rejane.	
Data: 	2018-06-01	
Ano: 	2018	
Palavras-chave: 	Porcine circovirus type 2 (PCV2); Capsid protein; Molecular modeling and antigenic epitope.	
Resumo: 	Abstract Economic losses with high mortality rate associated with Porcine circovirus type 2 (PCV2) is reported worldwide. PCV2 commercial vaccine was introduced in 2006 in U.S. and in 2008 in Brazil. Although PCV2 vaccines have been widely used, cases of PCV2 systemic disease have been reported in the last years. Eleven nursery or fattening pigs suffering from PCV2 systemic disease were selected from eight PCV2-vaccinated farms with historical records of PCV2 systemic disease in Southern Brazil. PCV2 genomes were amplified and sequenced from lymph node samples of selected pigs. The comparison among the ORF2 amino acid sequences of PCV2 isolates revealed three amino acid substitutions in the positions F57I, N178S and A190T, respectively. Using molecular modeling, a structural model for the capsid protein of PCV2 was built. Afterwards, the mutated residues positions were identified in the model. The structural analysis of the mutated residues showed that the external residue 190 is close to an important predicted region for antibodies recognition. Therefore, changes in the viral protein conformation might lead to an inefficient antibody binding and this could be a relevant mechanism underlying the recent vaccine failures observed in swine farms in Brazil.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200351	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1016/j.bjm.2017.08.007	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.49 n.2 2018	
Fonte: 	info:eu-repo/semantics/openAccess