Data/hora: 01/06/2024 10:49:27

Provedor de dados: | 58
País: | Brazil
Título: | Molecular genetic differentiation of avian Escherichia coli by RAPD-PCR
Autores: | Salehi,Taghi Zahraei; Madani,Seyed Ahmad; Karimi,Vahid; Khazaeli,Fatemeh Arab.
Data: | 2008-09-01
Ano: | 2008
Palavras-chave: | Escherichia coli; RAPD-PCR; Molecular differentiation; Iran.
Resumo: | Escherichia coli is one of the most important bacterial avian pathogens and a common inhabitant of the gastrointestinal tract of animals. Most pathogenic E. coli can not be differentiated biochemically or by classic microbiologic methods. Molecular typing methods, particularly PCR, facilitated epidemiological and ecological studies of bacteria. Here we describe the application of a random amplified polymorphic DNA- polymerase chain reaction (RAPD-PCR) for molecular genetic differentiation of E. coli isolates in Iran. In this study 58 E. coli isolates including 4 standard strains, 3 food originated isolates, 33 avian isolates, 8 isolates form diarrheic calves and 10 isolates from unweaned diarrheic lambs were analyzed by RAPD-PCR using primer 1247(5/-AAG AGC CCG T-3/). The RAPD analysis showed that these isolates could be grouped into 33 RAPD types and avian isolates were discriminated into 29 genotypes. It was shown that the primer could not differentiate E. coli isolated from lambs. Discriminatory index for entire isolates was 0.912 and for avian isolates was 0.990. We concluded that RAPD-PCR can be used as a method for molecular differentiation of E. coli isolates.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300015
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822008000300015
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.39 n.3 2008
Fonte: | info:eu-repo/semantics/openAccess