Data/hora: 01/06/2024 11:49:51

Provedor de dados: | 58
País: | Brazil
Título: | A RT-PCR assay for the detection of encephalomycarditis virus infections in pigs
Autores: | Pérez,Lester J.; Díaz de Arce,Heidy.
Data: | 2009-12-01
Ano: | 2009
Palavras-chave: | Encephalomyocarditis virus; RT-PCR assay; Pig.
Resumo: | Encephalomyocarditis virus (EMCV) infections can cause losses in pig farms all over the world. Rapid, sensitive and unequivocal detection of this virus is therefore essential for the diagnosis and control of the disease. An RT-PCR assay was developed, optimized and evaluated for encephalomyocarditis virus detection in organ based on a pair of primers that amplifies a 165 bp DNA fragment from a highly conserved nucleotide region of the viral 3D glycoprotein. PCR products of the expected size were obtained from Cuban EMCV 744/03 strain. Non-specific reactions were not observed when other porcine RNA genome viruses and uninfected cells were used. The analytical sensitivity of the test was estimated to be 2 TCID50/50 mL. The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400034
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822009000400034
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.40 n.4 2009
Fonte: | info:eu-repo/semantics/openAccess