Data/hora: 01/06/2024 06:12:22

Provedor de dados: | 58
País: | Brazil
Título: | Different methods to quantify Listeria monocytogenesbiofilms cells showed different profile in their viability
Autores: | Winkelströter,Lizziane Kretli; Martinis,Elaine C.P. De.
Data: | 2015-03-01
Ano: | 2015
Palavras-chave: | Biofilms; L. monocytogenes; QPCR; Peroxyacetic acid; Sodium hypochlorite.
Resumo: | Listeria monocytogenes is a foodborne pathogen able to adhere and to form biofilms in several materials commonly present in food processing plants. The aim of this study was to evaluate the resistance of Listeria monocytogenes attached to abiotic surface, after treatment with sanitizers, by culture method, microscopy and Quantitative Real Time Polymerase Chain Reaction (qPCR). Biofilms of L. monocytogenes were obtained in stainless steel coupons immersed in Brain Heart Infusion Broth, under agitation at 37 °C for 24 h. The methods selected for this study were based on plate count, microscopic count with the aid of viability dyes (CTC-DAPI), and qPCR. Results of culture method showed that peroxyacetic acid was efficient to kill sessile L. monocytogenes populations, while sodium hypochlorite was only partially effective to kill attached L. monocytogenes (p &lt; 0.05). When, viability dyes (CTC/DAPI) combined with fluorescence microscopy and qPCR were used and lower counts were found after treatments (p &lt; 0.05). Selective quantification of viable cells of L. monocytogenes by qPCR using EMA revelead that the pre-treatment with EMA was not appropriate since it also inhibited amplification of DNA from live cells by ca. 2 log. Thus, the use of CTC counts was the best method to count viable cells in biofilms.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100231
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-838220131071
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.46 n.1 2015
Fonte: | info:eu-repo/semantics/openAccess