Data/hora: 01/06/2024 07:58:18

Provedor de dados: | 58
País: | Brazil
Título: | EMJH medium with 5-fluorouracil and nalidixic acid associated with serial dilution technique used to recover Leptospira spp from experimentally contaminated bovine semen
Autores: | Miraglia,Fabiana; Moraes,Zenaide Maria de; Melville,Priscilla Anne; Dias,Ricardo Augusto; Vasconcellos,Silvio Arruda.
Data: | 2009-03-01
Ano: | 2009
Palavras-chave: | Leptospires; Culture media; Antimicrobial; Isolation; Semen.
Resumo: | Bovine semen experimentally contaminated with Leptospira santarosai serovar Guaricura was submitted to the modified EMJH medium with 5-fluorouracil (300mg/L) and nalidixic acid (20mg/L), named as "selective medium" and using the serial dilution technique, in order to evaluate the percentage of recovery of the added microorganism. The selective EMJH medium was found with higher percentage of recovery of leptospiras and minor losses of samples due to contamination with opportunistic microorganisms than the non-selective EMJH medium: 151/376 (40.0%) of positive growth; and 38/376 (10.0%) contamination and 58/376 (15%) and 129/376 (34.0%), respectively. These results were statistically significant (p&lt;0. 0001; Fisher). Differences were found when the frequencies of positive leptospires recovery have been compared in the serial dilution technique (10-1 to 10-4) between the selective and non-selective media at different dilution factors. At 1/10th dilution the percentages found were (0%, 0/80) and (38%, 30/80), at 1/100th dilution, (3%, 2/80) and (49%, 39/80) and at 1/1,000th dilution, (25%, 20/80) and (50%, 40/80), respectively. The percentage of recovery of leptospires was found to be directly proportional to the dilution used. The methodology of the serial dilution technique (setting at least three dilutions) and the use of selective EMJH medium have been found to be efficient for the isolation of leptospires from the bovine semen samples.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100033
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822009000100033
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.40 n.1 2009
Fonte: | info:eu-repo/semantics/openAccess