Data/hora: 02/06/2024 04:34:16

Provedor de dados: | 58
País: | Brazil
Título: | Bovine Herpesvirus 4 in Parana State, Brazil: case report, viral isolation, and molecular identification
Autores: | Kruger,Ernesto Renato; Penha,Tania Regina; Stoffelo,Daura Regina Eira; Roehe,Paulo Michel; Ribeiro,Magda Costa; Soccol,Vanete Thomaz.
Data: | 2015-03-01
Ano: | 2015
Palavras-chave: | BoHV-4 virus; PCR; RFLP; Fingerprint.
Resumo: | Bovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinaesub-family and belongs to genus Rhadinovirus. This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. The goal of this work was to characterize a virus strain isolate from a cow’s uterine outflow. From swabs drawn of uterine secretion, a virus strain was isolated and characterized by its cytopathology, morphology, and molecular biology approaches. In culture there was CPE development, characterized mainly by long strands with several small balloons along them, radiated from infected cells. Electron microscopy analysis revealed virus particles that had icosahedrical capsid symmetry surrounded by a loose envelope, typical of a herpesvirus. A 2,571 bp PCR product after HindIII digestion generated four fragments, whose base pair composition were 403, 420, 535, and 1,125 bp. Restriction enzymes HindIII and BamHI generated the expected diagnostic bands as well as a 2,350 bp hypermolar fragment as a result of BamHI treatment to demonstrate that agent was a bovine herpesvirus 4, appertaining to DN-599 group.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100279
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-838246120130949
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.46 n.1 2015
Fonte: | info:eu-repo/semantics/openAccess