Data/hora: 11/06/2024 01:00:26

Provedor de dados: 	58	
País: 	Brazil	
Título: 	In vitro establishment and propagation of a Brazilian strain ofAnaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells	
Autores: 	Bastos,Camila V.; Passos,Lygia M. F.; Vasconcelos,Maria Mercês C.; Ribeiro,Múcio F. B.	
Data: 	2009-06-01	
Ano: 	2009	
Palavras-chave: 	IDE8 cells; Anaplasma marginale; In vitro culture.	
Resumo: 	A Brazilian isolate of Anaplasma marginale with appendage was successfully established and maintained in vitro in a tick cell line (IDE8). Infection was confirmed by optical and transmission electron microscopy. In addition, primers MSP1aNF2 and MSP1aNR2 amplified products from DNA extracted from infected IDE8 cells. Comparisons with partial sequences of the msp1α gene and the complete genome of A. marginale confirmed that the sequences of amplified fragments were from the A. marginale genome. This is the first establishment of a Brazilian A. marginale isolate in tick cells, representing a new system for biological and molecular studies and also a new source of material for diagnosis and development of vaccines.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200034	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822009000200034	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.40 n.2 2009	
Fonte: 	info:eu-repo/semantics/openAccess