Data/hora: 09/06/2024 13:25:39

Provedor de dados: | 58
País: | Brazil
Título: | Protease with collagenolytic activity produced by Bacillus sp. DPUA 1728 from Amazonian soil
Autores: | Lima,Lorena A.; Cruz Filho,Raimundo F.; Santos,Januário G. dos; Silva,Wilson C.
Data: | 2015-12-01
Ano: | 2015
Palavras-chave: | Collagenolytic protease; Bacillus sp; Factorial design; Amazonian soil.
Resumo: | Qualitative analyses were carried out on solid medium with insoluble collagen 0.25% (w/v) to detect proteases with collagenolytic activity produced by Bacillus sp. In cultures incubated for 24 h, a 23 full factorial design with four repetitions at the center point was developed to analyze the effects and interactions between initial pH, temperature and the concentration of gelatin. Based on the results of the first 23 full factorial design, a successive 23 full factorial design was performed. The most favorable production conditions were found to be 1.5% (w/v) gelatin, pH 9.0 and 37 °C with enzymatic activity of 86.27 U/mL. The enzyme showed optimal activity at 50 °C and pH 9.0, and it was stable over wide pH (7.2-10.0) and temperature (45 °C-60 °C) ranges. These results indicate that Bacillus sp DPUA 1728 is a potential source for producing collagenolytic protease with possible biotechnological applications, such as in the food, cosmetics and leather industries.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401217
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822015005030656
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.46 n.4 2015
Fonte: | info:eu-repo/semantics/openAccess