Data/hora: 06/06/2024 04:33:55

Provedor de dados: | 58
País: | Brazil
Título: | Comparison of DNA-extraction methods and Selective Enrichment broths on the detection of Salmonella Typhimurium in swine feces by polymerase chain reaction (PCR)
Autores: | Freschi,Carla Roberta; Carvalho,Luiz Fernando de Oliveira e Silva; Oliveira,Celso José Bruno de.
Data: | 2005-12-01
Ano: | 2005
Palavras-chave: | Diagnosis; DNA; Feces; PCR; Salmonella Typhimurium.
Resumo: | The aim of this study was to compare different DNA-extraction methods and selective enrichment broths for their effectiveness to detect Salmonella Typhimurium in artificially inoculated swine feces samples (100 CFU/g) by polymerase chain reaction. After enrichment in Rappaport-Vassiliadis, selenite cystine or Müller-Kauffmann tetrathionate, aliquots were used for DNA extraction by three different methods: boiling-centrifugation, phenol-chloroform and salting-out. Aliquots of extracted DNA were then used as template in PCR. The selective enrichment broths had no effect on the efficiency of PCR when boiling-centrifugation and salting-out were used. On the other hand, phenol-chloroform was superior (P&lt;0.05) when combined to Rappaport-Vassiliadis. Considering cost and efficiency parameters, we encourage the use of Müller-Kauffmann tetrathionate broth in combination with boiling-centrifugation DNA-extraction procedure.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400011
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822005000400011
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.36 n.4 2005
Fonte: | info:eu-repo/semantics/openAccess