Data/hora: 11/06/2024 00:31:16

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Comparison of DNA-extraction methods and Selective Enrichment broths on the detection of Salmonella Typhimurium in swine feces by polymerase chain reaction (PCR)	
Autores: 	Freschi,Carla Roberta; Carvalho,Luiz Fernando de Oliveira e Silva; Oliveira,Celso José Bruno de.	
Data: 	2005-12-01	
Ano: 	2005	
Palavras-chave: 	Diagnosis; DNA; Feces; PCR; Salmonella Typhimurium.	
Resumo: 	The aim of this study was to compare different DNA-extraction methods and selective enrichment broths for their effectiveness to detect Salmonella Typhimurium in artificially inoculated swine feces samples (100 CFU/g) by polymerase chain reaction. After enrichment in Rappaport-Vassiliadis, selenite cystine or Müller-Kauffmann tetrathionate, aliquots were used for DNA extraction by three different methods: boiling-centrifugation, phenol-chloroform and salting-out. Aliquots of extracted DNA were then used as template in PCR. The selective enrichment broths had no effect on the efficiency of PCR when boiling-centrifugation and salting-out were used. On the other hand, phenol-chloroform was superior (P&lt;0.05) when combined to Rappaport-Vassiliadis. Considering cost and efficiency parameters, we encourage the use of Müller-Kauffmann tetrathionate broth in combination with boiling-centrifugation DNA-extraction procedure.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400011	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822005000400011	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.36 n.4 2005	
Fonte: 	info:eu-repo/semantics/openAccess