Data/hora: 01/06/2024 00:55:00

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Chitinase Production by Streptomyces sp. ANU 6277	
Autores: 	Narayana,Kolla J.P.; Vijayalakshmi,Muvva.	
Data: 	2009-12-01	
Ano: 	2009	
Palavras-chave: 	Chitinase; Streptomyces sp. ANU 6277; Optimization; Characterization.	
Resumo: 	Chitinase production by a terrestrial Streptomyces sp. ANU 6277 was studied under sub-merged fermentation. Chitinase production started after 24 h of incubation and reached maximum levels after 60 h of cultivation. A high level of chitinase activity was observed in the culture medium with pH 6 at 35ºC. Culture medium amended with 1% chitin was found to be suitable for maximum production of chitinase. An optimum concentration of colloidal chitin for chitinase production was determined. Studies on the influence of additional carbon and nitrogen sources on chitinase production revealed that starch and yeast extract served as good carbon and nitrogen sources to enhance chitinase yield.Chitinase was purified from crude enzyme extract by single step gel filtration by Sephadex G-100. Purified chitinase of the strain exhibited a distinct protein band near 45 kDa by means of SDS-PAGE.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400002	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822009000400002	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.40 n.4 2009	
Fonte: 	info:eu-repo/semantics/openAccess