Data/hora: 14/06/2024 04:14:30

Provedor de dados: | 58
País: | Brazil
Título: | Expression of mouse beta defensin 2 in Escherichia coli and its broad-spectrum antimicrobial activity
Autores: | Gong,Tianxiang; Li,Wanyi; Wang,Yueling; Jiang,Yan; Zhang,Qiang; Feng,Wei; Jiang,Zhonghua; Li,Mingyuan.
Data: | 2011-09-01
Ano: | 2011
Palavras-chave: | Mouse beta defensin 2; Expression; Purification; Antimicrobial activity.
Resumo: | Mature mouse beta defensin 2 (mBD2) is a small cationic peptide with antimicrobial activity. Here we established a prokaryotic expression vector containing the cDNA of mature mBD2 fused with thioredoxin (TrxA), pET32a-mBD2. The vector was transformed into Escherichia Coli (E. coli) Rosseta-gami (2) for expression fusion protein. Under the optimization of fermentation parameters: induce with 0.6 mM isopropylthiogalactoside (IPTG) at 34ºC in 2×YT medium and harvest at 6 h postinduction, fusion protein TrxA-mBD2 was high expressed in the soluble fraction (&gt;95%). After cleaved fusion protein by enterokinase, soluble mature mBD2 was achieved 6 mg/L with a volumetric productivity. Purified recombinant mBD2 demonstrated clear broad-spectrum antimicrobial activity for fungi, bacteria and virus. The MIC of antibacterial activity of against Staphylococcus aureus was 50 µg/ml. The MIC of against Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans) was 12.5µg/ml and 25µg/ml, respectively. Also, the antimicrobial activity of mBD2 was effected by NaCl concentration. Additionally, mBD2 showed antiviral activity against influenza A virus (IAV), the protective rate for Madin-Darby canine kidney cells (MDCK) was 93.86% at the mBD2 concentration of 100 µg/ml. These works might provide a foundation for the following research on the mBD2 as therapeutic agent for medical microbes.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300043
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822011000300043
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.42 n.3 2011
Fonte: | info:eu-repo/semantics/openAccess