Data/hora: 11/06/2024 09:25:26

Provedor de dados: | 58
País: | Brazil
Título: | Treatment of serum with supernatants from cultures of Candida albicans reduces its serum-dependent phagocytosis
Autores: | Santos,Aderbal Antonio dos; Sá,Eglius Alexandre Colignese de; Gaziri,Luis Carlos Jabur; Felipe,Ionice.
Data: | 2002-01-01
Ano: | 2002
Palavras-chave: | Candida albicans; Phagocytosis; Proteinase.
Resumo: | Candida albicans is a potent activator of the complement system, and heat labile opsonins produced by activation of C3 (C3b and iC3b) enhance phagocytosis of C. albicans mediated by complement receptors. In this study we treated mouse serum with supernatants from cultures of a protease producer strain of C. albicans and evaluated the ability of this serum to enhance phagocytosis of C. albicans. Cell-free supernatants from cultures of C. albicans were concentrated 5 fold and added to mouse serum for 30 min at 37ºC, before using this serum for opsonization of glutaraldehyde-fixed yeast cells. We observed that normal mouse serum increased about 3 fold the phagocytosis of C. albicans by mice peritoneal macrophages, whereas supernatant-treated serum did not increase phagocytosis. This effect of supernatants on serum was prevented by addition of pepstatin (5 µg/ ml; an inhibitor of C. albicans acid proteases) to the medium. Serum treated with supernatants from cultures of a protease-deficient mutant of C. albicans also increased about 3 fold phagocytosis of the yeast. These results suggest that a protease produced by C. albicans causes proteolysis of serum opsonins, thereby reducing the phagocytosis of the yeast.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100016
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822002000100016
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.33 n.1 2002
Fonte: | info:eu-repo/semantics/openAccess