Data/hora: 05/06/2024 18:06:41

Provedor de dados: 	58	
País: 	Brazil	
Título: 	ExoU contributes to late killing of Pseudomonas aeruginosa - infected endothelial cells	
Autores: 	Saliba,Alessandra Mattos; Nishi,Rebeca; Assis,Maria Cristina; Plotkowski,Maria Cristina.	
Data: 	2003-11-01	
Ano: 	2003	
Palavras-chave: 	Type III secretion system; ExoU; Endothelial cells; Bacterial cytotoxicity.	
Resumo: 	To ascertain the role of ExoU in late P. aeruginosa cytotoxicity, endothelial cells (EC) were exposed to wild type PA103, PA103deltaexoU and PA103::exsA for 1h and to gentamicin in culture medium. After 24h, the viability of PA103-infected cells (33.7 ± 14.3%) was significantly lower than the viability of PA103deltaexoU- (77.7 ± 6.3%) or PA103::exsA- (79.5 ± 23.3%) infected EC. P. aeruginosa cytotoxicity did not depend on the bacterial ability to interact with EC because the percentage of cells with associated PA103 (35.9 ± 15.8%) was similar to the percentage in PA103deltaexoU- (34.2 ± 16.0%) and lower than the percentage in PA103::exsA-infected cultures (82.9 ± 18.9%). Cell treatment with cytochalasin D reduced the PA103 internalization by EC but did not interfere with its ability to kill host cells.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500008	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822003000500008	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.34 suppl.1 2003	
Fonte: 	info:eu-repo/semantics/openAccess