Data/hora: 07/06/2024 09:46:31

Provedor de dados: | 58
País: | Brazil
Título: | A novel expression vector for the secretion of abaecin in Bacillus subtilis
Autores: | Li,Li; Mu,Lan; Wang,Xiaojuan; Yu,Jingfeng; Hu,Ruiping; Li,Zhen.
Data: | 2017-12-01
Ano: | 2017
Palavras-chave: | Abaecin; Antimicrobial peptides; Bacillus subtilis; Expression.
Resumo: | ABSTRACT This study aimed to describe a Bacillus subtilis expression system based on genetically modified B. subtilis. Abaecin, an antimicrobial peptide obtained from Apis mellifera, can enhance the effect of pore-forming peptides from other species on the inhibition of bacterial growth. For the exogenous expression, the abaecin gene was fused with a tobacco etch virus protease cleavage site, a promoter Pglv, and a mature beta-glucanase signal peptide. Also, a B. subtilis expression system was constructed. The recombinant abaecin gene was expressed and purified as a recombinant protein in the culture supernatant. The purified abaecin did not inhibit the growth of Escherichia coli strain K88. Cecropin A and hymenoptaecin exhibited potent bactericidal activities at concentrations of 1 and 1.5 µM. Combinatorial assays revealed that cecropin A and hymenoptaecin had sublethal concentrations of 0.3 and 0.5 µM. This potentiating functional interaction represents a promising therapeutic strategy. It provides an opportunity to address the rising threat of multidrug-resistant pathogens that are recalcitrant to conventional antibiotics.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400809
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1016/j.bjm.2017.01.009
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.48 n.4 2017
Fonte: | info:eu-repo/semantics/openAccess