Data/hora: 12/06/2024 13:24:59

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Polyclonal anti-intimin antibody: immunological characterization and its use in EPEC and EHEC diagnosis	
Autores: 	Koga,Paula Célia Mariko; Menezes,Caroline Anunciação; Lima,Flávia Afonso; Nara,Júlia Mitico; Magalhães,Caroline Arantes; Cianciarullo,Aurora Marques; Ferreira-Júnior,Jorge Mario da Costa; Trabulsi,Luiz Rachid; Mendes-Ledesma,Meire Roberta Bresciani; Piazza,Roxane Maria Fontes.	
Data: 	2003-11-01	
Ano: 	2003	
Palavras-chave: 	Intimin; Phenotypical analysis; Polyclonal antibody; EPEC; EHEC.	
Resumo: 	Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesion (A/E). Through immunoblotting, immunofluorescence, flow citometry and immunogold we observed that the obtained polyclonal antibody against conserved intimin region recognizes the different intimin subtypes and suggests that it can be used as a tool for EPEC and EHEC detection. Besides, immuno-dot assay seems to be a possible alternative as a capture method.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500002	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822003000500002	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.34 suppl.1 2003	
Fonte: 	info:eu-repo/semantics/openAccess