Data/hora: 05/06/2024 01:04:58

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Highly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp. AB68	
Autores: 	Aygan,Ashabil; Arikan,Burhan; Korkmaz,Hatice; Dinçer,Sadik; Çolak,Ömer.	
Data: 	2008-09-01	
Ano: 	2008	
Palavras-chave: 	Bacillus sp.; Α-amylase; Alkaliphilic; Thermostable; Enzyme.	
Resumo: 	An alkaliphilic and highly thermostable α-amylase producing Bacillus sp. was isolated from Van soda lake. Enzyme synthesis occurred at temperatures between 25ºC and 40ºC. Analysis of the enzyme by SDS-PAGE revealed a single band which was estimated to be 66 kDa. The enzyme was active in a broad temperature range, between 20ºC and 90ºC, with an optimum at 50ºC; and maximum activity was at pH 10.5. The enzyme was almost completely stable up to 80ºC with a remaining activity over 90% after 30 min pre-incubation. Thermostability was not increased in the presence of Ca2+. An average of 75% and 60ºC of remaining activity was observed when the enzyme was incubated between pH 5 and 9 for 1 h and for 2 h, respectively. The activity of the enzyme was inhibited by SDS and EDTA by 38% and 34%, respectively.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300027	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822008000300027	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.39 n.3 2008	
Fonte: 	info:eu-repo/semantics/openAccess