Data/hora: 02/06/2024 14:40:30

Provedor de dados: | 58
País: | Brazil
Título: | Detection of Leptospira spp. in the semen and urine of bulls serologically reactive to Leptospira interrogans serovar hardjo
Autores: | Magajevski,Fernanda Senter; Girio,Raul Jose Silva; Mathias,Luis Antonio; Myashiro,Simone; Genovez,Margareth Élide; Scarcelli,Eliana Pinheiro.
Data: | 2005-03-01
Ano: | 2005
Palavras-chave: | Semen; Urine; PCR.
Resumo: | This study evaluated PCR for the detection of leptospires in semen and urine of ten serologically reactive bulls, comparing these results with those obtained with other diagnosis techniques. Two collections of materials were done in alternate days. Semen and urine samples were separated in aliquots for: direct visualization in dark field microscopy; inoculation in hamsters (for semen only); isolation in culture media; and PCR. No hamster was positive by the microscopic agglutination test (MAT); kidney and liver fragments from the hamsters were used in an isolation attempt in culture media, with one positive isolation from the kidney of a hamster inoculated with semen of one bull, and from liver of hamsters inoculated with semen of three bulls. Isolation in culture was negative for all semen samples, but positive for five urine samples by direct inoculation. In PCR there was no positive result for semen samples, and only one urine sample was positive, which was coincident with one of the positive cultures. It was not possible to visualize leptospires in any of the samples by dark field microscopy.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100009
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822005000100009
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.36 n.1 2005
Fonte: | info:eu-repo/semantics/openAccess