Data/hora: 06/06/2024 00:59:03

Provedor de dados: 	58	
País: 	Brazil	
Título: 	NaCl stress impact on the key enzymes in glycolysis from Lactobacillus bulgaricus during freeze-drying	
Autores: 	Li,Chun; Sun,Jinwei; Qi,Xiaoxi; Liu,Libo.	
Data: 	2015-12-01	
Ano: 	2015	
Palavras-chave: 	Lactobacillus bulgaricus; Freeze-drying; NaCl stress; Semi-quantitative RT-PCR; Glycolysis.	
Resumo: 	Abstract The viability of Lactobacillus bulgaricus in freeze-drying is of significant commercial interest to dairy industries. In the study, L.bulgaricus demonstrated a significantly improved (p &lt; 0.05) survival rate during freeze-drying when subjected to a pre-stressed period under the conditions of 2% (w/v) NaCl for 2 h in the late growth phase. The main energy source for the life activity of lactic acid bacteria is related to the glycolytic pathway. To investigate the phenomenon of this stress-related viability improvement in L. bulgaricus, the activities and corresponding genes of key enzymes in glycolysis during 2% NaCl stress were studied. NaCl stress significantly enhanced (p &lt; 0.05) glucose utilization. The activities of glycolytic enzymes (phosphofructokinase, pyruvate kinase, and lactate dehydrogenase) decreased during freeze-drying, and NaCl stress were found to improve activities of these enzymes before and after freeze-drying. However, a transcriptional analysis of the corresponding genes suggested that the effect of NaCl stress on the expression of the pfk2 gene was not obvious. The increased survival of freeze-dried cells of L. bulgaricus under NaCl stress might be due to changes in only the activity or translation level of these enzymes in different environmental conditions but have no relation to their mRNA transcription level.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401193	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-838246420140595	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.46 n.4 2015	
Fonte: 	info:eu-repo/semantics/openAccess