Data/hora: 05/06/2024 17:11:58

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Detection of Salmonella spp, Salmonella Enteritidis and Typhimurium in naturally infected broiler chickens by a multiplex PCR-based assay	
Autores: 	Paião,F.G.; Arisitides,L.G.A.; Murate,L.S.; Vilas-Bôas,G.T.; Vilas-Boas,L.A.; Shimokomaki,M.	
Data: 	2013-01-01	
Ano: 	2013	
Palavras-chave: 	Aviculture; Bacteria; Detection.	
Resumo: 	The presence of Salmonella in the intestinal tract, on the chickens skin and among their feathers, may cause carcasses contamination during slaughtering and processing and possibly it is responsible by the introduction of this microorganism in the slaughterhouses. A rapid method to identify and monitor Salmonella and their sorovars in farm is becoming necessary. A pre-enriched multiplex polymerase chain reaction (m-PCR) assay employing specific primers was developed and used to detect Salmonella at the genus level and to identify the Salmonella enterica serovar Enteritidis (S. Enteritidis) and Salmonella enterica serovar Typhimurium (S. Typhimurium) in broiler chicken swab samples. The method was validated by testing DNA extract from 90 fresh culture cloacal swab samples from poultry chicken cultured in phosphate buffer peptone water at 37 ºC for 18 h. The final results showed the presence of Salmonella spp. in 25% of samples, S. Enteritidis was present in 12% of the Salmonella-positive samples and S. Typhimurium in 3% of the samples. The m-PCR assay developed in this study is a specific and rapid alternative method for the identification of Salmonella spp. and allowed the observation of specific serovar contamination in the field conditions within the locations where these chickens are typically raised.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100005	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822013005000002	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.44 n.1 2013	
Fonte: 	info:eu-repo/semantics/openAccess