Data/hora: 01/06/2024 09:57:54

Provedor de dados: | 58
País: | Brazil
Título: | Selection of reference genes for expression analysis in the entomophthoralean fungus Pandora neoaphidis
Autores: | Chen,Chun; Xie,Tingna; Ye,Sudan; Jensen,Annette Bruun; Eilenberg,J ørgen.
Data: | 2016-03-01
Ano: | 2016
Palavras-chave: | Pandora neoaphidis; Reference genes; Quantitative PCR.
Resumo: | Abstract The selection of suitable reference genes is crucial for accurate quantification of gene expression and can add to our understanding of host–pathogen interactions. To identify suitable reference genes in Pandora neoaphidis, an obligate aphid pathogenic fungus, the expression of three traditional candidate genes including 18S rRNA(18S), 28S rRNA(28S) and elongation factor 1 alpha-like protein (EF1), were measured by quantitative polymerase chain reaction at different developmental stages (conidia, conidia with germ tubes, short hyphae and elongated hyphae), and under different nutritional conditions. We calculated the expression stability of candidate reference genes using four algorithms including geNorm, NormFinder, BestKeeper and Delta Ct. The analysis results revealed that the comprehensive ranking of candidate reference genes from the most stable to the least stable was 18S (1.189), 28S (1.414) and EF1 (3). The 18S was, therefore, the most suitable reference gene for real-time RT-PCR analysis of gene expression under all conditions. These results will support further studies on gene expression in P. neoaphidis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100259
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1016/j.bjm.2015.11.031
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.47 n.1 2016
Fonte: | info:eu-repo/semantics/openAccess