Data/hora: 06/06/2024 05:37:47

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans 191	
Autores: 	Fleuri,Luciana F.; Kawaguti,Haroldo Y.; Sato,Hélia H.	
Data: 	2009-09-01	
Ano: 	2009	
Palavras-chave: 	Chitinase; Cellulosimicrobium cellulans; Fungal lysis; Protoplasts.	
Resumo: 	This study concerned the production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans strain 191. In shaken flasks the maximum yield of chitinase was 6.9 U/mL after 72 h of cultivation at 25ºC and 200 rpm. In a 5 L fermenter with 1.5 vvm aeration, the highest yield obtained was 4.19 U/mL after 168 h of fermentation at 25ºC and 200 rpm, and using 3 vvm, it was 4.38 U/mL after 144 h of fermentation. The chitinase (61 KDa) was purified about 6.65 times by Sepharose CL 4B 200 gel filtration with a yield of 46.61%. The purified enzyme was able to lyse the cell walls of some fungi and to form protoplasts.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300026	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822009000300026	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.40 n.3 2009	
Fonte: 	info:eu-repo/semantics/openAccess