Data/hora: 09/06/2024 10:26:12

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Isolation and purification of complex II from proteus mirabilis strain ATCC 29245	
Autores: 	Shabbiri,Khadija; Ahmad,Waqar; Syed,Quratulain; Adnan,Ahmad.	
Data: 	2010-10-01	
Ano: 	2010	
Palavras-chave: 	Proteus mirabilis; Complex II; Cytochrome b.	
Resumo: 	A respiratory complex was isolated from plasma membrane of pathogenic Proteus mirabilis strain ATCC 29245. It was identified as complex II consisting of succinate:quinone oxidoreductase (EC 1.3.5.1) containing single heme b. The complex II was purified by ion-exchange chromatography and gel filtration. The molecular weight of purified complex was 116.5 kDa and it was composed of three subunits with molecular weights of 19 kDa, 29 kDa and 68.5 kDa. The complex II contained 9.5 nmoles of cytochrome b per mg protein. Heme staining indicated that the 19 kDa subunit was cytochrome b. Its reduced form showed absorptions peaks at 557.0, 524.8 and 424.4 nm. The α-band was shifted from 557.0 nm to 556.8 nm in pyridine ferrohemochrome spectrum. The succinate: quinone oxidoreductase activity was found to be high in this microorganism.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300032	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822010005000004	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.41 n.3 2010	
Fonte: 	info:eu-repo/semantics/openAccess