Data/hora: 12/06/2024 12:28:20

Provedor de dados: | 58
País: | Brazil
Título: | Application of statistical experimental design for optimisation of bioinsecticides production by sporeless Bacillus thuringiensis strain on cheap medium
Autores: | Ben Khedher,Saoussen; Jaoua,Samir; Zouari,Nabil.
Data: | 2013-09-01
Ano: | 2013
Palavras-chave: | Sporeless Bacillus thuringiensis; Delta-endotoxins; Cheap medium; Optimisation; Response surface methodology.
Resumo: | In order to overproduce bioinsecticides production by a sporeless Bacillus thuringiensis strain, an optimal composition of a cheap medium was defined using a response surface methodology. In a first step, a Plackett-Burman design used to evaluate the effects of eight medium components on delta-endotoxin production showed that starch, soya bean and sodium chloride exhibited significant effects on bioinsecticides production. In a second step, these parameters were selected for further optimisation by central composite design. The obtained results revealed that the optimum culture medium for delta-endotoxin production consists of 30 g L-1 starch, 30 g L-1 soya bean and 9g L-1 sodium chloride. When compared to the basal production medium, an improvement in delta-endotoxin production up to 50% was noted. Moreover, relative toxin yield of sporeless Bacillus thuringiensis S22 was improved markedly by using optimised cheap medium (148.5 mg delta-endotoxins per g starch) when compared to the yield obtained in the basal medium (94.46 mg delta-endotoxins per g starch). Therefore, the use of optimised culture cheap medium appeared to be a good alternative for a low cost production of sporeless Bacillus thuringiensis bioinsecticides at industrial scale which is of great importance in practical point of view.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300043
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822013000300043
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.44 n.3 2013
Fonte: | info:eu-repo/semantics/openAccess