Data/hora: 12/06/2024 17:40:42

Provedor de dados: 	58	
País: 	Brazil	
Título: 	A quantitative analysis of Propionibacterium acnes in lesional and non-lesional skin of patients with progressive macular hypomelanosis by real-time polymerase chain reaction	
Autores: 	Cavalcanti,Silvana Maria de Morais; França,Emmanuel Rodrigues de; Magalhães,Marcelo; Lins,Ana Kelly; Brandão,Laura Costa; Magalhães,Vera.	
Data: 	2011-06-01	
Ano: 	2011	
Palavras-chave: 	Hypopigmentation/progressive macular hypomelanosis; Propionibacterium acnes; Polymerase chain reaction; Biopsy/bacteriology; ROC curve.	
Resumo: 	Little is known about the etiology of progressive macular hypomelanosis, although it has been suggested that Propionibacterium acnes plays an important role. While microbiological culture is commonly employed to identify Propionibacterium acnes, new identification methods have been under investigation, amongst them polymerase chain reaction. To determine the cut-off point for the number of genome copies of Propionibacterium acnes in the lesional skin of patients with progressive macular hypomelanosis as a positive marker, employing quantitative real-time polymerase chain reaction and anaerobic culture, considered gold standard. An observational study with a comparison group, included 35 patients with dermatosis, attended at the Oswaldo Cruz University Hospital, Pernambuco, Brazil, between March and May 2008. Lesional skin was compared to non-lesional skin through positive testing with real-time polymerase chain reaction and culture. The Statistical Package for Social Sciences, version 12.0, was employed for the association analysis with the McNemar test, and the cut-off point with the ROC curve for maximum values. Propionibacterium acnes was most frequently encountered in lesional areas (p&lt;0,025). The cut-off point of Propionibacterium acnes in lesional skin was 1,333 genome copies, with a sensitivity of 87,9% and a specificity of 100,0%. Since Propionibacterium acnes is a saprophyte, identifying the cut-off point may assist in determining its positivity in lesional skin in patients suffering with this dermatosis.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200002	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822011000200002	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.42 n.2 2011	
Fonte: 	info:eu-repo/semantics/openAccess