Data/hora: 14/06/2024 03:44:39

Provedor de dados: | 58
País: | Brazil
Título: | Partial purification and characterization of exopolygalacturonase II and III of Penicillium frequentans
Autores: | Barense,Renata I.; Chellegatti,Maria Angélica dos S.C.; Fonseca,Maria José V.; Said,Suraia.
Data: | 2001-12-01
Ano: | 2001
Palavras-chave: | Exopolygalacturonase; Pectinase; Polygalacturonase; Penicillium frequentans.
Resumo: | Previous studies from our laboratory have demonstrated that the fungus Penicillium frequentans produces high levels of polygalacturonase and pectinesterase. Endopolygalacturonase I (Endo-PG I) and Exopolygalacturonase I (Exo-PG I) were previously purified and characterized. In the present study two extracellular polygalacturonases were separated, partially purified and biochemically characterized. Both were characterized as exopolygalacturonases so they were named exopolygalacturonase II (Exo-PG II) and exopolygalacturonase III (Exo-PG III) which had a molecular mass of 63 kDa (Exo-PG II) and 79 kDa (Exo-PG III). The Km values were 1.6 and 0.059 g/L and the Vmax values were 2571 and 185 U/mg, respectively. The optimum temperature was 50ºC for both enzymes, while the optimum pH was 5.0 for Exo-PG II and 5.8 for Exo-PG III.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400014
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822001000400014
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.32 n.4 2001
Fonte: | info:eu-repo/semantics/openAccess