Data/hora: 06/06/2024 00:49:26

Provedor de dados: | 58
País: | Brazil
Título: | A novel method to optimize culture conditions for biomass and sporulation of the entomopathogenic fungus Beauveria Bassiana IBC1201
Autores: | Gao,Li.
Data: | 2011-12-01
Ano: | 2011
Palavras-chave: | Nutrition; Environment; Biomass; Sporulation; Beauveria bassiana.
Resumo: | Biomass yields and sporulation of Beauveria bassiana was concerned on culture conditions, environmental factors and cultivation method. We optimized the best culture conditions for biomass yields of B. bassiana IBC1201 with the novel "two-stage" cultivation method as well as orthogonal matrix method. Firstly, we cultured spore suspension on the basal medium (sucrose 19.00 g, soy peptone 4.06 g, K2HPO4 1.00 g, KCl 0.50 g, MgSO4 0.50 g, FeSO4 0.10 g and 17.00 g Bactor) for the first stage culture of 4 days under room condition. Then, we transferred them to another defined medium (Cellobiose 9.52 g, urea 1.70 g, ZnSO4•7H2O 0.05 g/L, MnSO4•H2O 0.005 g/L, CaCl2 1.00 g/L, CuSO4•5H2O 0.05 g/L and 17.00 g Bactor) for more 4 days cultivation with the environmental factors combination of water potential -1.2 MPa /pH 3 /12 h light cycle/ 23 ℃ for biomass yields, and with the environmental factors combination of water potential -0.8 MPa /pH 3 /24 h light cycle/ 23 ℃ for spore yields. These results provided important information for mass production (including biomass and spore yields) of this great potential biocontrol fungus.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400045
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822011000400045
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.42 n.4 2011
Fonte: | info:eu-repo/semantics/openAccess