Data/hora: 06/06/2024 10:19:14

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Assessment of the pathogenicity of cell-culture-adapted Newcastle disease virus strain Komarov	
Autores: 	Visnuvinayagam,Sivam; K,Thangavel; N,Lalitha; S,Malmarugan; Sukumar,Kuppannan.	
Data: 	2015-09-01	
Ano: 	2015	
Palavras-chave: 	Newcastle disease; Komarov strain; Chicken embryo fibroblast; Newcastle disease vaccine.	
Resumo: 	Newcastle disease vaccines hitherto in vogue are produced from embryonated chicken eggs. Egg-adapted mesogenic vaccines possess several drawbacks such as paralysis and mortality in 2-week-old chicks and reduced egg production in the egg-laying flock. Owing to these possible drawbacks, we attempted to reduce the vaccine virulence for safe vaccination by adapting the virus in a chicken embryo fibroblast cell culture (CEFCC) system. Eighteen passages were carried out by CEFCC, and the pathogenicity was assessed on the basis of the mean death time, intracerebral pathogenicity index, and intravenous pathogenicity index, at equal passage intervals. Although the reduction in virulence demonstrated with increasing passage levels in CEFCC was encouraging, 20% of the 2-week-old birds showed paralytic symptoms with the virus vaccine from the 18th(final) passage. Thus, a tissue-culture-adapted vaccine would demand a few more passages by CEFCC in order to achieve a complete reduction in virulence for use as a safe and effective vaccine, especially among younger chicks. Moreover, it can be safely administered even to unprimed 8-week-old birds.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300861	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-838246320140051	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.46 n.3 2015	
Fonte: 	info:eu-repo/semantics/openAccess