Data/hora: 05/06/2024 01:08:18

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Capture immunoassay for LT detection produced by enterotoxigenic Escherichia coli in bacterial isolates	
Autores: 	Menezes,Caroline Anunciação; Gonçalves,Danielle Silva; Amianti,Jackeline; Fernandes,Irene; Taddei,Carla Romano; Koga,Paula Célia Mariko; Trabulsi,Luiz Rachid; Martinez,Marina Baquerizo; Piazza,Roxane Maria Fontes.	
Data: 	2003-11-01	
Ano: 	2003	
Palavras-chave: 	Detection; Polyclonal antibody; Monoclonal antibody; Thermo-labile toxin; E. coli.	
Resumo: 	A capture enzyme-linked immunosorbent assay (ELISA), which detects LT-I toxin produced by enterotoxigenic Escherichia coli strains, has beendeveloped. This capture assay was performed using the IgG enriched fraction of anti-LT-I antiserum and IgG2b anti-LT-I monoclonal antibody and allowed a clear distinction between E. coli LT-I - producing and non-producing strains. The estimated accuracy of the assay is 78% for sensitivity, 94% for specificity and 92% for efficiency. Thus, the capture immunoassayis a sensitive tool for detection of E. coli, which produces heat-labile enterotoxin, and is suitable for use in clinical laboratories and epidemiological surveys in developing world.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500004	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822003000500004	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.34 suppl.1 2003	
Fonte: 	info:eu-repo/semantics/openAccess