Data/hora: 05/06/2024 01:52:07

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Direct identification of bovine mastitis pathogens by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in pre-incubated milk	
Autores: 	Barreiro,Juliana R.; Gonçalves,Juliano L.; Grenfell,Rafaella; Leite,Renata F.; Juliano,Luiz; Santos,Marcos V.	
Data: 	2018-12-01	
Ano: 	2018	
Palavras-chave: 	Milk; Bacteria; Mastitis; Mass spectrometry.	
Resumo: 	ABSTRACT The present study aimed to compare two MALDI-TOF identification methods [(a) direct sample identification after pre-incubation; or (b) use of bacteria isolated on pre-culture)] to standard, traditional bench microbiology. A total of 120 quarter milk samples from 40 Holstein lactating cows were screened based on culture-positive results obtained by microbiological culture (reference method) with the following numbers of quarters positive per cow: 4 cows with 1, 8 cows with 2, 12 cows with 3 and 16 cows with 4 infected quarters per cow. For direct identification method, quarter milk samples (n = 120) were skimmed by centrifugation (10,000 × g/10 min) and pre-incubated at 37 ºC for 12 h. After pre-incubation, quarter milk samples were submitted to total bacterial count by flow cytometry and for a preparation protocol for bacterial ribosomal protein extraction followed by MALDI-TOF MS analysis. The direct MALDI-TOF MS identification method compared to microbiological culture correctly identified isolates of coagulase-negative Staphylococci (27.2%), Streptococcus agalactiae (21.8%), Staphylococcus aureus (14.2%), and Streptococcus uberis (5.2%). The pre-incubation protocol of milk samples, associated to the direct identification method by MALDI-TOF MS, did not increase the identification at species level (score &gt;2.0) of pathogens causing subclinical mastitis in comparison to the method without previous incubation.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400801	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1016/j.bjm.2018.04.012	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.49 n.4 2018	
Fonte: 	info:eu-repo/semantics/openAccess