Data/hora: 05/06/2024 03:40:43

Provedor de dados: | 58
País: | Brazil
Título: | Antifungal activity of different neem leaf extracts and the nimonol against some important human pathogens
Autores: | Mahmoud,D.A; Hassanein,N.M; Youssef,K.A; Abou Zeid,M.A.
Data: | 2011-09-01
Ano: | 2011
Palavras-chave: | Azadirachta indica; Aqueous and organic extracts; HPLC; Fungal inhibitory effect.
Resumo: | This study was conducted to evaluate the effect of aqueous, ethanolic and ethyl acetate extracts from neem leaves on growth of some human pathogens (Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus terreus, Candida albicans and Microsporum gypseum) in vitro. Different concentrations (5, 10, 15 and 20%) prepared from these extracts inhibited the growth of the test pathogens and the effect gradually increased with concentration. The 20% ethyl acetate extract gave the strongest inhibition compared with the activity obtained by the same concentration of the other extracts. High Performance Liquid Chromatography (HPLC) analysis of ethyl acetate extract showed the presence of a main component (nimonol) which was purified and chemically confirmed by Nuclear Magnetic Resonance (NMR) spectroscopic analysis. The 20% ethyl acetate extract lost a part of its antifungal effect after pooling out the nimonol and this loss in activity was variable on test pathogens. The purified nimonol as a separate compound did not show any antifungal activity when assayed against all the six fungal pathogens.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300021
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822011000300021
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.42 n.3 2011
Fonte: | info:eu-repo/semantics/openAccess