Data/hora: 09/06/2024 14:36:50

Provedor de dados: | 58
País: | Brazil
Título: | Isolation and characterization of an Ashbya gossypii mutant for improved riboflavin production
Autores: | Wei,Shiping; Hurley,James; Jiang,Zhenglong; Wang,Siwen; Wang,Yuanyuan.
Data: | 2012-06-01
Ano: | 2012
Palavras-chave: | Riboflavin; Ashbya gossypii; Ultraviolet irradiation; Medium optimization.
Resumo: | The use of the filamentous fungus, Ashbya gossypii, to improve riboflavin production at an industrial scale is described in this paper. A riboflavin overproducing strain was isolated by ultraviolet irradiation. Ten minutes after spore suspensions of A. gossypii were irradiated by ultraviolet light, a survival rate of 5.5% spores was observed, with 10% of the surviving spores giving rise to riboflavin-overproducing mutants. At this time point, a stable mutant of the wild strain was isolated. Riboflavin production of the mutant was two fold higher than that of the wild strain in flask culture. When the mutant was growing on the optimized medium, maximum riboflavin production could reach 6.38 g/l. It has even greater promise to increase its riboflavin production through dynamic analysis of its growth phase parameters, and riboflavin production could reach 8.12 g/l with pH was adjusted to the range of 6.0-7.0 using KH2PO4 in the later growth phase. This mutant has the potential to be used for industrial scale riboflavin production.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200003
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822012000200003
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.43 n.2 2012
Fonte: | info:eu-repo/semantics/openAccess