Data/hora: 05/06/2024 17:45:48

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Rhizobacterial characterization for quality control of eucalyptus biogrowth promoter products	
Autores: 	Zarpelon,Talyta Galafassi; Guimarães,Lúcio Mauro da Silva; Alfenas-Zerbini,Poliane; Lopes,Eli Sidney; Mafia,Reginaldo Gonçalves; Alfenas,Acelino Couto.	
Data: 	2016-12-01	
Ano: 	2016	
Palavras-chave: 	Formulation; PGPR; Eucalyptus; Biological product.	
Resumo: 	Abstract Plant growth-promoting rhizobacteria strains from special formulations have been used to optimize eucalyptus cutting production. To undertake quality control for the formulated products, the rhizobacterial strains should be characterized to assess their purity and authentication. In the present study, we characterized nine strains of rhizobacteria, including three Bacillus subtilis (S1, S2 and 3918), two Pseudomonas sp. (MF4 and FL2), P. putida (MF2), P. fulva (Ca), Frateuria aurantia (R1), and Stenotrophomonas maltophilia (CIIb). The strains were differentiated by colony morphology after 24 h of incubation in three different solid state culture media (glucose-nutritive agar, 523 medium and yeast extract-mannitol agar), sensitivity to a panel of 28 antibiotics (expressed according to the formation of inhibition halos of bacterial growth in the presence of antibiotics), and PCR-RFLP profiles of the 16S rDNA gene produced using nine restriction enzymes. It was possible to differentiate all nine strains of rhizobacteria using their morphological characteristics and sensitivity to antibiotics. The molecular analysis allowed us to separate the strains CIIb, FL2 and R1 from the strains belonging to the genera Bacillus and Pseudomonas. By using these three methods concomitantly, we were able to determine strain purity and perform the authentication.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400973	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1016/j.bjm.2016.07.013	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.47 n.4 2016	
Fonte: 	info:eu-repo/semantics/openAccess