Data/hora: 07/06/2024 02:40:21

Provedor de dados: 	58	
País: 	Brazil	
Título: 	In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections	
Autores: 	Zauli,Danielle Alves Gomes; Menezes,Carla Lisandre Paula de; Oliveira,Cristiane Lommez de; Mateo,Elvis Cristian Cueva; Ferreira,Alessandro Clayton de Souza.	
Data: 	2016-12-01	
Ano: 	2016	
Palavras-chave: 	QPCR; Hepatitis B; Hepatitis C.	
Resumo: 	Abstract The quantification of viral nucleic acids in serum by real-time PCR plays an important role in diagnosing hepatitis B virus and hepatitis C virus infection. In this study, we developed an assay using specific primers and probes to quantify hepatitis B virus DNA or hepatitis C virus RNA in serum from infected patients. For standardization and validation of the assay, an international panel of hepatitis B virus/hepatitis C virus and standard plasmids was used. A correlation coefficient of 0.983 and 0.963 for hepatitis B virus and hepatitis C virus, respectively, was obtained based on cycle threshold values and concentrations of DNA or RNA. The standard curve showed a linear relationship from 19 IU/mL to 1.9 × 109 IU/mL of serum, with a coefficient of determination (r2) of 0.99. In sera from patients infected with hepatitis B virus or hepatitis C virus viral loads (19 IU/mL and 1.9 × 109 IU/mL), we quantified viral loads with a detection limit of 1.9 × 102 IU/mL. The real-time quantitative PCR assay developed in this study provides an ideal system for routine diagnosis and confirmation of indeterminate serological results, especially in immunosuppressed patients.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400987	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1016/j.bjm.2016.07.008	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.47 n.4 2016	
Fonte: 	info:eu-repo/semantics/openAccess