Data/hora: 07/06/2024 10:23:23

Provedor de dados: | 58
País: | Brazil
Título: | In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
Autores: | Zauli,Danielle Alves Gomes; Menezes,Carla Lisandre Paula de; Oliveira,Cristiane Lommez de; Mateo,Elvis Cristian Cueva; Ferreira,Alessandro Clayton de Souza.
Data: | 2016-12-01
Ano: | 2016
Palavras-chave: | QPCR; Hepatitis B; Hepatitis C.
Resumo: | Abstract The quantification of viral nucleic acids in serum by real-time PCR plays an important role in diagnosing hepatitis B virus and hepatitis C virus infection. In this study, we developed an assay using specific primers and probes to quantify hepatitis B virus DNA or hepatitis C virus RNA in serum from infected patients. For standardization and validation of the assay, an international panel of hepatitis B virus/hepatitis C virus and standard plasmids was used. A correlation coefficient of 0.983 and 0.963 for hepatitis B virus and hepatitis C virus, respectively, was obtained based on cycle threshold values and concentrations of DNA or RNA. The standard curve showed a linear relationship from 19 IU/mL to 1.9 × 109 IU/mL of serum, with a coefficient of determination (r2) of 0.99. In sera from patients infected with hepatitis B virus or hepatitis C virus viral loads (19 IU/mL and 1.9 × 109 IU/mL), we quantified viral loads with a detection limit of 1.9 × 102 IU/mL. The real-time quantitative PCR assay developed in this study provides an ideal system for routine diagnosis and confirmation of indeterminate serological results, especially in immunosuppressed patients.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400987
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1016/j.bjm.2016.07.008
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.47 n.4 2016
Fonte: | info:eu-repo/semantics/openAccess