Data/hora: 06/06/2024 08:08:05

Provedor de dados: | 58
País: | Brazil
Título: | Detection of Mycoplasma pulmonis in laboratory rats
Autores: | Barreto,Maria Lucia; Nascimento,Elmiro Rosendo do; Campos,Carlos Augusto de Martino; Nascimento,Maria da Graça Fichel do; Lignon,Gilberto Brasil; Lira,Marie Luce Flores; Silva,Ricardo G.
Data: | 2002-09-01
Ano: | 2002
Palavras-chave: | Mycoplasma pulmonis; Diagnosis; Isolation; Serology; Rats.
Resumo: | This work was conducted on rats in two premises located in Niterói and Rio de Janeiro, Brazil. One is classified as conventional controlled and the other, conventional. The objective of the present study was to detect the presence of Mycoplasma pulmonis in animals with symptoms of respiratory disease and low reproductive performance. In the conventional controlled premises, 16 rats of Wistar-Furth strain were necropsied while in the conventional premises necropsy was performed on 12 rats of Hooded Lister strain. The clinical samples of lungs, trachea, oropharynx, middle ear, uterus and ovaries were subjected to culturing while the sera were tested for antibody detection. From 28 rats, 57.14% (16/28) were culture positive for M. pulmonis, being 81.25% (13/16) from the conventional controlled premises, and 25.00% (3/12) from the conventional premises. The ELISA test was carried out in 20 animals of both colonies. In the conventional controlled premises, 92.86% (13/14) were positive for M. pulmonis, and 7.14% (1/14) were suspicious, while in the conventional premises, 100% (6/6) of the samples were positive. The results confirmed that M. pulmonis was the etiologic agent of the disease that affected the rats under study, and that the ELISA positivity rated higher than culture.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300015
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822002000300015
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.33 n.3 2002
Fonte: | info:eu-repo/semantics/openAccess