Data/hora: 03/06/2024 06:29:13

Provedor de dados: | 58
País: | Brazil
Título: | Adhesion and invasion of Clostridium perfringens type A into epithelial cells
Autores: | Llanco,Luis A.; Nakano,Viviane; Moraes,Claudia T.P. de; Piazza,Roxane M.F.; Avila-Campos,Mario J.
Data: | 2017-12-01
Ano: | 2017
Palavras-chave: | Adhesion; Invasion; Clostridium perfringens type A; Necrotic enteritis; TpeL gene.
Resumo: | ABSTRACT Clostridium perfringens is the causative agent for necrotic enteritis. It secretes the major virulence factors, and α- and NetB-toxins that are responsible for intestinal lesions. The TpeL toxin affects cell morphology by producing myonecrosis, but its role in the pathogenesis of necrotic enteritis is unclear. In this study, the presence of netB and tpeL genes in C. perfringens type A strains isolated from chickens with necrotic enteritis, their cytotoxic effects and role in adhesion and invasion of epithelial cells were evaluated. Six (27.3%) of the 22 C. perfringens type A strains were harboring the tpeL gene and produced morphological alterations in Vero cells after 6 h of incubation. Strains tpeL (-) induced strong cell rounding after 6 h of incubation and produced cell enlargement. None of the 22 strains harbored netB gene. All the six tpeL (+) gene strains were able to adhere to HEp-2 cells; however, only four of them (66.6%) were invasive. Thus, these results suggest that the presence of tpeL gene or TpeL toxin might be required for the adherence of bacteria to HEp-2 cells; however, it could not have any role in the invasion process.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400764
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1016/j.bjm.2017.06.002
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.48 n.4 2017
Fonte: | info:eu-repo/semantics/openAccess